Quantitative Analysis

Assay of Acid Phosphatase enzyme activity from Potatoes

Assay of Acid Phosphatase enzyme activity

This is the enzymology protocol on “Assay of Acid phosphatase enzyme activity from Potatoes”. Before going to the protocol, we should know few points about the phosphatase enzyme. A phosphatase is an enzyme that removes a phosphate group from its substrate by hydrolyzing phosphoric acid monoesters into a phosphate ion and a molecule with a free hydroxyl group.This action is directly opposite to that of phosphorylases and kinases, which attach phosphate groups to their substrates by using energetic molecules like ATP.A common phosphatase …

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Assay of Urease Enzyme Activity (Enzymology Practical Protocol)

Assay of Urease Enzyme activity Protocol

This is the Biochemistry Protocols section post. Here we are providing the “Assay of Urease enzyme activity” protocol. before going to the protocol we need to know a few points about “Urease Enzyme”. Assay of Urease Enzyme Activity What is Urease? Urease is a hydrolytic enzyme that catalyzes the Urea into Carbon dioxide and ammonia. The Enzyme Commission Number is …

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Effect of Temperature on Amylase activity (Enzymology Protocol)

Effect of Temperature on Amylase enzyme activity

Aim: To study the effect of temperature on enzyme activity and to determine the optimum temperature. Principle: In any enzyme catalyze reaction the velocity increases with temperature till it reaches the maximum the velocity decreases with further increase of temperature this effect of temperature may be due to several reasons. An effect on the stability of the enzymeAn effect on …

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Assay of Salivary Amylase enzyme activity

Assay of amylase enzyme activity

This article providing information on “Salivary amylase, functions, and Assay of Salivary amylase activity”. An enzyme is a protein molecule that is a biological catalyst with three characteristics. The basic function of an enzyme is to increase the rate of a reaction.Most enzymes act specifically with only one reactant, called a substrate, to produce products.The most remarkable characteristic is that enzymes are regulated from a state of low activity …

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Determination of Carbohydrate by Anthrone Method

Anthrone method

Carbohydrates are a very important component of Storage and structural materials in the plants. The carbohydrates are stored as free sugars and polysaccharides.  The basic units of carbohydrates are Monosaccharides. When hydrolyzing the carbohydrates, it gives monosaccharides, but when hydrolyzing monosaccharides it cannot be split into simpler sugars. The hydrolyzed product of Polysaccharide is estimating by the resultant monosaccharides. Determination of Carbohydrate by …

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Estimation of Proteins by Lowry method (Quantitative Analysis)

Estimation of Proteins by Lowry method (Quantitative Analysis) - Biochemistry Den

Aim: To estimate the concentration of proteins by the Lowry method from the given sample. Principle The Blue color developed by the reduction of the Phosphomolybdic-Phosphotungastic components in the Folin-ciocalteau reagent by the amino acids Tyrosine and Tryptophan present in the protein plus the color developed by the biuret reaction of the protein with the color developed by the biuret reaction …

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Estimation of Tyrosine by Folin-Ciocalteau Method

Estimation of Tyrosine by Folin-Ciocalteau Method

Are you looking for the procedure of Estimation of Tyrosine? Here is the complete biochemical protocol with a downloadable document. The Folin–Ciocalteu reagent (FCR) or Folin’s phenol reagent or Folin–Denis reagent, also called the Gallic Acid Equivalence method (GAE), is a mixture of phosphomolybdate and phosphotungstate used for the colourimetric in vitro assay of phenolic and polyphenolic antioxidants. It is …

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