Protocols

Estimation of Blood Glucose level by Folin-Wu method

Estimation of Blood Glucose level by Folin-Wu method

Estimation of Blood Glucose level by the Folin-Wu method is one of the clinical biochemistry protocols. You can download these protocols from the given link. What is the Folin-Wu tube? The modification of the Folin and Wu method for quantitative blood sugar determination used in this laboratory consists merely in: 1. Diluting the unknown after the addition of the phosphomolybdate-phosphotungstate …

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Assay of Acid Phosphatase enzyme activity from Potatoes

Assay of Acid Phosphatase enzyme activity

This is the enzymology protocol on “Assay of Acid phosphatase enzyme activity from Potatoes”. Before going to the protocol, we should know few points about the phosphatase enzyme. A phosphatase is an enzyme that removes a phosphate group from its substrate by hydrolyzing phosphoric acid monoesters into a phosphate ion and a molecule with a free hydroxyl group.This action is directly opposite to that of phosphorylases and kinases, which attach phosphate groups to their substrates by using energetic molecules like ATP.A common phosphatase …

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Assay of Urease Enzyme Activity (Enzymology Practical Protocol)

Assay of Urease Enzyme activity Protocol

This is the Biochemistry Protocols section post. Here we are providing the “Assay of Urease enzyme activity” protocol. before going to the protocol we need to know a few points about “Urease Enzyme”. Assay of Urease Enzyme Activity What is Urease? Urease is a hydrolytic enzyme that catalyzes the Urea into Carbon dioxide and ammonia. The Enzyme Commission Number is …

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Effect of Temperature on Amylase activity (Enzymology Protocol)

Effect of Temperature on Amylase enzyme activity

Aim: To study the effect of temperature on enzyme activity and to determine the optimum temperature. Principle: In any enzyme catalyze reaction the velocity increases with temperature till it reaches the maximum the velocity decreases with further increase of temperature this effect of temperature may be due to several reasons. An effect on the stability of the enzymeAn effect on …

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Assay of Salivary Amylase enzyme activity

Assay of amylase enzyme activity

This article providing information on “Salivary amylase, functions, and Assay of Salivary amylase activity”. An enzyme is a protein molecule that is a biological catalyst with three characteristics. The basic function of an enzyme is to increase the rate of a reaction.Most enzymes act specifically with only one reactant, called a substrate, to produce products.The most remarkable characteristic is that enzymes are regulated from a state of low activity …

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Determination of Carbohydrate by Anthrone Method

Anthrone method

Carbohydrates are a very important component of Storage and structural materials in the plants. The carbohydrates are stored as free sugars and polysaccharides.  The basic units of carbohydrates are Monosaccharides. When hydrolyzing the carbohydrates, it gives monosaccharides, but when hydrolyzing monosaccharides it cannot be split into simpler sugars. The hydrolyzed product of Polysaccharide is estimating by the resultant monosaccharides. Determination of Carbohydrate by …

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Qualitative Analysis: Color Reactions of Lipids

color reactions of Lipids

Are you searching for the protocol on Color reactions of Lipids? Lipids are one of the major constituents of foods and are important in our diet for a number of reasons. They are a major source of energy and provide essential lipid nutrients. Nevertheless, over-consumption of certain lipid components can be detrimental to our health, e.g. cholesterol and saturated fats. …

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What are the Basic Color reactions of Amino acids?

Color Reactions of amino acids

Are you looking for the color reactions of amino acids? Biochemistry Den presents the student with selected chemical reactions of the Proteins and some of the amino acids with makeup proteins. These compounds are essential components of living organisms, including the human body, and the range of tests available shows how much time scientists have devoted to this branch of …

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