Amino Acids Classification and its Basics

Amino acids are organic compounds that contain an amino group (-NH₂) and a carboxyl group (-COOH) attached to a central carbon atom. They are the building blocks of proteins and perform various functions in living organisms.

There are about 500 amino acids known, but only 20 are encoded by the genetic code and used to synthesize proteins.

These 20 amino acids can be classified based on different criteria, such as the structure, polarity, charge, and function of their side chains (R groups).

This article will introduce the basic concepts of amino acid classification and its importance for understanding protein structure and function.

The proteins on hydrolysis yield mixtures of the component amino acids.

Therefore, to understand the structure and chemistry of proteins, we have to first undertake the study of amino acids.

Structure of Amino acid

Amino acids are the building blocks of proteins and have a common structure with some variations. The basic structure of an amino acid consists of a central carbon atom (called the alpha carbon) that is bonded to four different groups: a hydrogen atom, a carboxyl group (-COOH), an amino group (-NH₂), and a variable group (called the R-group or side chain).

The R-group is what distinguishes one amino acid from another and determines its chemical properties. Amino acids can be classified into four categories based on their R-groups: nonpolar, polar, acidic, or basic.

• Nonpolar amino acids have hydrophobic R-groups that do not interact with water.
• Polar amino acids have hydrophilic R-groups that can form hydrogen bonds with water or other polar molecules.
• Acidic amino acids have R-groups that contain a carboxyl group (-COOH) that can donate a proton and become negatively charged.
• Basic amino acids have R-groups that contain an amino group (-NH₂) that can accept a proton and become positively charged.
• Amino acids exist in a zwitterionic form, meaning they have both positive and negative charges on different atoms, at physiological pH.

Amino acids Classification

It can classify amino acids into 4 types:

1. Classification based on the position of “-NH₂”
2. Classification based on the composition of the “-R’ side chain
3. Classification based on the Nutritional requirement
4. Classification based on the Metabolic Fate

1. Classification based on the position of “-NH₂”

They can classify amino acids into THREE types:

i. α-amino acid

The amino group attached to the next carbon of the carboxyl group is called “α-amino acid” All naturally occurring amino acids are in “α-L-amino acids”.

ii. β-amino acid

The amino group attached to the third carbon (numbering from the Carboxyl group) of the amino acid is called “β-amino acid”. Eg: β-alanine, is one of the end products of Pyrimidine catabolism.

iii) γ-amino acid

The amino group attached to the Fourth carbon (numbering from the Carboxyl group) of the amino acid is called “γ-amino acid”. Eg: GABA (Gamma Amino Butyric Acid)

2. Classification based on the composition of “-R’ side chain

Based on the composition of the ‘R’ side chain, amino acids can be categorized into 8 types: (Fairly & Kigour, 1966)

a) Neutral Amino Acids (or) Simple amino acids

These have no functional group in the side chain. Eg:

b) Hydroxyl Group containing amino acids

These contain a hydroxyl group in their side chain. Eg:

c) Sulphur Containing amino acids

These possess a sulfur atom in the side chain. Eg:

d) Acidic amino acids

These have a Carboxyl group in the side chain

e) Basic amino acids

These possess an amino group in the side chain. Eg:

f) Heterocyclic amino acid

These amino acids have in their side chain a ring that possesses at least one atom other than carbon. Eg:

g) Aromatic amino acid

These have a benzene ring in the side chain. Eg:

h) Imino acid

These are also heterocyclic compounds, which have an “imino group” (-NH-) instead of an amino group (-NH2).

3. Classification based on the Nutritional requirement

Based on Nutritional requirements, amino acids can be divided into 3 types.

1. Essential Amino acids (EAA)
2. Non-Essential Amino acids (NEAA)
3. Semi-Essential Amino acids (SEAA)

1. Essential Amino acids (EAA)

Some amino acids don’t synthesize in the human body. It should be supplied through diet. They are required for the proper growth and maintenance of the individual.

Eg:

MATT VIL PHLy

(or)

PVT TIM HALL

M= Methionine A=Arginine T=Threonine T=Tryptophan V=Valine

I=Isoleucine L=Leucine P=Phenylalanine H=Histidine L=Lysine

2. Non-Essential Amino acids (NEAA)

The body can synthesize about 10 amino acids to meet biological needs, hence they need not be consumed in the diet.

Eg: Gly, Ala, Ser, Cys, Asp, Asn, Glu, Gln, Tyr, and Pro.

3. Semi-Essential Amino acid

Histidine and Arginine are semi-essential amino acids. Growing children require them in food. But they are not essential for the adult individual. 

4. Classification based on the Metabolic Fates

In this Amino acids classification,  it can be classified based on the basis of metabolic fate:

1. Purely ketogenic amino acids
2. Ketogenic and Glucogenic amino acids
3. Purely Glucogenic amino acids

i. Purely ketogenic amino acids

Leucine is purely ketogenic because it is converted into ketone bodies.

ii. Ketogenic and Glucogenic amino acids

During metabolism, part of the carbon skeleton of these amino acids will enter the ketogenic pathway and the other part of the glucogenic pathway.

Eg: Lys, Ile, Phe, Tyr & Trp are partially ketogenic and partially glucogenic.

iii. Purely Glucogenic amino acids

All the remaining 14 amino acids are purely glucogenic as they enter only into the glucogenic pathway.

Reference: Structural Amino acids

Frequently Asked Questions (FAQs)

Additional readings:

• Physio-Chemical Properties of Amino Acids? (Guide)
• What is Amino acid and its Structural Chemistry?
• What are the Fundamental molecules of Proteins?

Conclusion

Amino acids are the basic building blocks of proteins and have various structures and functions. It can be classified based on the location of their functional groups, their polarity, their ionization, their side chain group type, their distribution in proteins, and their nutritional requirements.

Amino acids play important roles in biological processes such as metabolism, gene expression, enzyme catalysis, and signaling. These are essential nutrients for humans and other organisms and must be obtained from dietary sources or synthesized by the body.

Amino acids are also involved in many industrial applications such as food additives, pharmaceuticals, cosmetics, and biotechnology. So these are fascinating molecules that deserve further study and exploration.