Biochemical studies or biochemical techniques rely on the availability of appropriate analytical techniques and their applications. This undergraduate course deals with different biochemical techniques such as spectrophotometry and chromatography to gain knowledge of biomolecules such as proteins, lipids, carbohydrates, nucleic acids, and their functions.

Massive progress has recently been achieved in understanding gene structure and expression, and as a result, molecular biology and biochemistry are closely related in many ways. The molecular and immunological methods covered in this course, such as ELISA, radioimmunoassay, blotting, PCR, cell culture, hybridoma, and cloning processes, are primarily focused on diagnosing illnesses, malfunctions, and abnormalities in order to develop remedial solutions.

Molecular and immunological techniques such as ELISA, radioimmunoassay, blotting, PCR, cell culture, hybridoma, and cloning protocols dealt with in this course are mainly focused on understanding the diagnosis of diseases, malfunctions, and disorders to generate corrective measures.

Recently, huge advances have been made in understanding gene structure and its expression, and hence, the discipline of molecular biology overlaps with that of biochemistry in many aspects. Molecular and immunological techniques such as ELISA, radioimmunoassay, blotting, PCR, cell culture, hybridoma, and cloning protocols dealt with in this course are mainly focused on understanding the diagnosis of diseases, malfunctions, and disorders to generate corrective measures.

Molecular and immunological techniques such as ELISA, radioimmunoassay, blotting, PCR, cell culture, hybridoma, and cloning protocols dealt with in this course are mainly focused on understanding the diagnosis of diseases, malfunctions, and disorders to generate corrective measures.

basics of biochemical techniques

Basics of Biochemical Techniques

Some biochemical techniques are given here.

1. Biochemical Tests

These five tests identify the main biologically important chemical compounds. For each test, take a small amount of the substance to be tested and shake it in water in a test tube. If the sample is a piece of food, then grind it with some water in a pestle and mortar to break up the cells and release the cell contents. Many of these compounds are insoluble, but the tests work just as well on a fine suspension.

Starch (iodine test)

  • To approximately 2 cm3 of the test solution, add two drops of iodine/potassium iodide solution.
  • A blue-black color indicates the presence of starch as a starch-polyiodide complex is formed.
  • Starch is only slightly soluble in water, but the test works well in suspension or as a solid.

Reducing Sugars (Benedict’s test)

  • All monosaccharides and most disaccharides (except sucrose) will reduce copper (II) sulfate, producing a precipitate of copper (I) oxide on heating, so they are called reducing sugars.
  • Benedict’s reagent is an aqueous solution of copper (II) sulfate, sodium carbonate, and sodium citrate.
  • To approximately 2 cm3 of test solution, add an equal quantity of Benedict’s reagent. Shake, and heat for a few minutes at 95 °C in a water bath.
  • A precipitate indicates reducing sugar. The color and density of the precipitate indicate the amount of reducing sugar present, so this test is semi-quantitative.
  • A green precipitate means relatively little sugar; a brown or red precipitate means progressively more sugar is present.

Non-reducing Sugars (Benedict’s test)

  • Sucrose is called a non-reducing sugar because it does not reduce copper sulfate, so there is no direct test for sucrose.
  • However, if it is first hydrolyzed (broken down) to its constituent monosaccharides (glucose and fructose), it will then give a positive Benedict’s test.
  • So, sucrose is the only sugar that will give a negative Benedict’s test before hydrolysis and a positive test afterward. First test a sample for reducing sugars, to see if there are any present before hydrolysis. Then, using a separate sample, boil the test solution with dilute hydrochloric acid for a few minutes to hydrolyze the glycosidic bond. Neutralize the solution by gently adding small amounts of solid sodium hydrogen carbonate until it stops fizzing, then test as before for reducing sugars.
  • First test a sample for reducing sugars, to see if there are any present before hydrolysis. Then, using a separate sample, boil the test solution with dilute hydrochloric acid for a few minutes to hydrolyze the glycosidic bond. Neutralize the solution by gently adding small amounts of solid sodium hydrogen carbonate until it stops fizzing, then test as before for reducing sugars.

Lipids (emulsion test)

  • Lipids do not dissolve in water but do dissolve in ethanol. This characteristic is used in the emulsion test.
  • Do not start by dissolving the sample in water, but instead, shake some test samples with about 4 cm³ of ethanol.
  • Decant the liquid into a test tube of water, leaving any undissolved substances behind.
  • If there are lipids dissolved in the ethanol, they will precipitate in the water, forming a cloudy white emulsion.

Protein (biuret test)

  • To about 2 cm3 of the test, the solution adds an equal volume of biuret solution, down the side of the test tube.
  • A blue ring forms at the surface of the solution, which disappears on shaking, and the solution turns lilac-purple, indicating protein.
  • The color is due to a complex between nitrogen atoms in the peptide chain and Cu2+ ions, so this is really a test for peptide bonds.

2. Chromatography

Chromatography is used to separate pure substances from a mixture of substances, such as a cell extract. It is based on different substances having different solubility in different solvents. A simple and common form of chromatography use filter paper.

  1. Pour some solvent into a chromatography tank and seal it, so the atmosphere is saturated with solvent vapor. Different solvents are suitable for different tasks, but they are usually mixtures of water with organic liquids such as ethanol or propanone.
  2. Place a drop of the mixture to be separated onto a sheet of chromatography paper near one end. This is the origin of the chromatogram. The spot should be small but concentrated. Repeat this for any other mixtures. Label the spots with a pencil, as the ink may dissolve.
  3. Place the chromatography sheet into the tank so that the origin is just above the level of solvent, and leave it for several hours. The solvent will rise to the surface of the paper by capillary action, carrying the contents of the mixture with it. Any solutes dissolved in the solvent will be partitioned between the organic solvent (the moving phase) and the water, which is held by the paper (the stationary phase). The more soluble a solute is in the solvent, the further up the paper it will move.
  4. When the solvent has nearly reached the top of the paper, the paper is removed and the position of the solvent front is marked. The chromatogram may need to be developed to make the spots visible. For example, amino acids stain purple with ninhydrin.
  5. The chromatogram can be analyzed by measuring the distance traveled by the solvent front, and the distance from the origin to the center of each spot. This is used to calculate the Rf (relative front) value for each spot:

Rf value is characteristic of a particular solute in a particular solvent. It can be used to identify components of a mixture by comparing to tables of known Rf values.

chromatography 1

Sometimes chromatography with a single solvent is not enough to separate all the constituents of a mixture. In this case, the separation can be improved by two-dimensional chromatography, where the chromatography paper is turned to 90° and run a second time in a second solvent. Solutes that didn’t separate in one solvent will separate in another because they have different solubility.

chromatography 2

There are many different types of chromatography.

  • Paper chromatography is the simplest but does not always give a very clean separation.
  • Thin-layer chromatography (TLC) uses a thin layer of cellulose or silica-coated plastic or glass sheet. This is more expensive but gives much better and more reliable separation.
  • Column chromatography uses a glass column filled with a cellulose slurry. Large samples can be pumped through the column and the separated fractions can be collected for further experiments, so this is preparative chromatography as opposed to analytical chromatography.
  • High-performance liquid chromatography (HPLC) is an improved form of column chromatography that delivers excellent separation quickly.
  • Electrophoresis uses an electric current to separate molecules because of charge. It can also be used to separate because of molecular size, and as such, is used in DNA sequencing.

3. Cell Fractionation

 This means separating different parts and organelles of a cell so that they can be studied in detail. All the processes of cell metabolism (such as respiration or photosynthesis) have been studied in this way. This is one of the biochemical techniques. The most common method of fractionating cells is the use of differential centrifugation.

A more sophisticated separation can be performed by density gradient centrifugation. In this, the cell-free extract is centrifuged in a dense solution (such as sucrose or cesium chloride). The fractions don’t pellet, but instead, separate into layers with the densest fractions near the bottom of the tube. The desired layer can then be pipetted off. This is the technique used in the Meselson-Stahl experiment (module 2) and it is also used to separate the two types of ribosomes. The terms “70S” and “80S” refer to their positions in a density gradient.

4. Enzyme Kinetics

This means measuring the rate of enzyme reactions.

  • Firstly, you need a signal to measure that shows the progress of the reaction. The signal should change with either substrate or product concentration, and it should preferably be something that can be measured continuously. Typical signals include color changes, pH changes, mass changes, gas production, volume changes, or turbidity changes. If the reaction has none of these properties, it can sometimes be linked to a second reaction that does generate one of these changes.
  • If you mix your substrate with an enzyme and measure your signal, you will obtain a time course. If the signal is proportional to substrate concentration, it will start high and decrease, while if the signal is proportional to the product, it will start low and increase. In both cases, the time course will be curved (actually an exponential curve).
  • How do you obtain a rate from this time-course? One thing that is not a good idea is to measure the time taken for the reaction, for as the time-course shows, it is very difficult to say when the reaction ends: it just gradually approaches the end-point. A better method is to measure the initial rate, which is the initial slope of the time course. This also means you don’t need to record the whole time-course, but simply take one measurement a short time after mixing.
  • Repeat this initial rate measurement under different conditions (such as different substrate concentrations) and then plot a graph of rate vs. the factor. Each point on this second graph is taken from a separate initial rate measurement (or, better yet, it is an average of several initial rate measurements under the same conditions). Draw a smooth curve through the points.
  • Be careful not to confuse the two kinds of graphs (the time-course and rate graphs) when interpreting your data.
  • One useful trick is to dissolve the substrate in agar on an agar plate. If a source of the enzyme is placed on the agar plate, the enzyme will diffuse out through the agar, turning the substrate into the product as it goes. There must be a way to distinguish the substrate from the product, and the reaction will then show up as a ring around the enzyme source.
  • The higher the concentration of enzyme, the higher the diffusion gradient, so the faster the enzyme diffuses through the agar, the larger the ring at a given time.
  • The diameter of the ring is, therefore, proportional to the enzyme concentration. This can be done for many enzymes, e.g., a protein agar plate can be used for a protease enzyme, or a starch agar plate can be used for the enzyme amylase.

5. Microscopy :

Of all the techniques used in biology, microscopy is probably the most important. The vast majority of living organisms are too small to be seen in any detail with the human eye, and cells and their organelles can only be seen with the aid of a microscope. Cells were first seen in 1665 by Robert Hooke (who named them after monks’ cells in a monastery) and were studied in more detail by Leeuwenhoek using a primitive microscope.

Units of measurement. The standard SI units of measurement used in microscopy are:

  • metre (m)               = 1 m
  • millimetre (mm)   = 10-3 m
  • micrometre        = 10-6 m
  • nanometre (nm) = 10-9 m
  • picometer (pm)  = 10-12 m
  • angstrom (Å0)     = 10-10 m (obsolete)

Magnification and Resolving Power. By using more lenses microscopes can magnify by a larger amount, but this doesn’t always mean that more detail can be seen. The amount of detail depends on the resolving power of a microscope, which is the smallest separation at which two separate objects can be distinguished (or resolved). It is calculated by the formula:

where lambda is the wavelength of light, and n.a. is the numerical aperture of the lens (which ranges from about 0.5 to 1.4). So the resolving power of a microscope is ultimately limited by the wavelength of light (400-600nm for visible light). To improve the resolving power a shorter wavelength of light is needed, and sometimes microscopes have blue filters for this purpose (because blue has the shortest wavelength of visible light).

Different kinds of Microscope

Light Microscope:

  • This is the oldest, simplest, and most widely used form of microscopy.
  • Specimens are illuminated with light, which is focused using glass lenses and viewed using the eye or photographic film.
  • Specimens can be living or dead, but often need to be stained with a colored dye to make them visible.
  • Many strains are available that strain-specific parts of the cell, such as DNA, lipids, the cytoskeleton, etc.
  • All light microscopes today are compound microscopes, which means they use several lenses to obtain high magnification.
  • Light microscopy has a resolution of about 200 nm, which is good enough to see cells, but not the details of cell organelles.
  • There has been a recent resurgence in the use of light microscopy, partly due to technical improvements that have dramatically improved the resolution far beyond the theoretical limit. For example, fluorescence microscopy has a resolution of about 10 nm, while interference microscopy has a resolution of about 1 nm.

Electron Microscope

  • This uses a beam of electrons, rather than electromagnetic radiation, to “illuminate” the specimen.
  • This may seem strange, but electrons behave like waves and can easily be produced (using a hot wire), focused (using electromagnets), and detected (using a phosphor screen or photographic film).
  • A beam of electrons has an effective wavelength of less than 1 nm, so it can be used to resolve small subcellular ultrastructures.
  • The development of the electron microscope in the 1930s revolutionized biology, allowing organelles such as mitochondria, ER, and membranes to be seen in detail for the first time.

The main problem with the electron microscope is that specimens must be fixed in plastic and viewed in a vacuum, and must, therefore, be dead. Other issues are that the specimens can be damaged by the electron beam and that they must be stained with an electron-dense chemical (usually heavy metals like osmium, lead, or gold). Initially, there was a problem of artifacts (i.e. observed structures that were due to the preparation process and were not real), but improvements in technology have eliminated most of these.

There are two kinds of electron microscopes. The transmission electron microscope (TEM) works much like a light microscope, transmitting a beam of electrons through a thin specimen and then focusing the electrons to form an image on a screen or on film. This is the most common form of an electron microscope and has the best resolution. The scanning electron microscope (SEM) scans a fine beam of electrons onto a specimen and collects the electrons scattered across the surface. This has a poorer resolution but gives excellent 3-dimensional images of surfaces.

  • X-ray Microscope: This is an obvious improvement to the light microscope since x-rays have wavelengths a thousand times shorter than visible light, and so could even be used to resolve atoms. Unfortunately, there are no good x-ray lenses, so an image cannot be focused, and usable x-ray microscopes do not yet exist. However, x-rays can be used without focusing on giving a diffraction pattern, which can be used to work out the structures of molecules, such as those of proteins and DNA.
  • Scanning Tunneling Microscope (or Atomic Force Microscope): This uses a very fine needle to scan the surface of a specimen. It has a resolution of about 10 pm and has been used to observe individual atoms for the first time.

Comparison of Light and Electron Microscopes

types of microscopes table

Frequently Asked Questions (FAQs)

  1. What are biochemical techniques?

    Biochemical techniques are methods used in the laboratory to study biochemical processes. These techniques can be used to identify the chemicals involved in a biochemical reaction, measure the rate of a biochemical reaction, or determine the structure of a protein or other molecule.

  2. What are the biological techniques?

    Biological techniques are the use of biochemistry, microbiology, and genetics to study the genetic makeup of organisms.

  3. What are the analytical techniques?

    Analytical techniques involve the detection of a particular entity in a sample. It is important to have a good understanding of this because this technique is used often, and it helps identify our samples. Once identified, samples can then be further analyzed for specific contents such as cellular components, chemicals, and other compounds in those samples.

  4. Why do we study biological techniques?

    One reason is to better understand the natural world and how things work. For example, biologists might study techniques that allow them to track the movements of animals or parasites to better understand their biology or epidemiology.

  5. What are molecular genetic techniques?

    Molecular genetic techniques are used to study the structure and function of genes. These techniques include DNA sequencing, gene mapping, and gene expression analysis.

  6. What are the basic techniques in gene analysis?

    The techniques used in gene analysis can be broadly divided into two groups: those that change the sequence of DNA, and those that seek to identify the presence or absence of particular genes.

  7. What are biochemical examples?

    Examples of biochemical processes that occur in the human body are protein synthesis, DNA replication, and glycoprotein synthesis.

  8. What are the biological techniques?

    To study an organism in-depth, biologists often use biochemical approaches. Analytical techniques employ biochemical approaches to study organisms. These include a wide range of tests such as microbiological, enzymatic, and genetic analysis. Examples of analyses used to identify differences in bacterial populations from experiments done on mouse cells or human blood samples taken from an individual undergoing clinical care for kidney disease. Detecting increasing levels before damage occurs allows doctors to intervene ahead of time.

  9. What are biochemical examples?

    Examples of biochemical molecules are proteins, lipids, and nucleic acids.

  10. What are the biological techniques?

    Biologist uses biochemical approaches to study their organisms. For example, – allows scientists to communicate and critique each other’s work in a medium relevant to a wider audience and tag posts accordingly with different terms of reference that most people understand, including “presentation,” and even ones that may be unfamiliar, like “Non-transcriptional.” This type of approach reduces self-deception about an observation except as it is reflected in the reporter’s conclusions. Popularization increases understanding of biological phenomena and communication with groups that are otherwise inaccessible, such as the hostile public, but is just a short-term solution to issues arising from loss of confidence.

Final Words

Biochemical techniques are applied for the study of any biochemical process, it may be a metabolic pathway or a single enzyme or protein. Biochemical analysis is helpful for the study of the molecular basis of disease, biochemical pathways, and metabolism. The biochemical analysis mainly includes Enzyme assays, Spectroscopy, Immunoassays, HPLC, Mass Spectroscopy, Genetic Analysis, and DNA Fingerprinting.

Over the years, biochemistry has emerged as an indispensable tool in biological and medical sciences. It has made it possible to understand the underlying causes of several diseases and is used in the diagnosis and treatment of several types of illnesses. Biochemistry has also made it possible to develop new drugs and medicines. While there are various biochemical techniques, here are some of the basics.

The basics of biochemical techniques are discussed above with a few methods only. These are not advanced parts.

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