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Biochemical Techniques

Basics of Biochemical Techniques

Basics of Biochemical Techniques

Hours August 9, 2024August 9, 2024
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Table of Contents

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  • A. Spectroscopy
    • 1. UV-Visible Spectroscopy
    • 2. Fluorescence Spectroscopy
    • 3. Infrared (IR) Spectroscopy
    • 4. Nuclear Magnetic Resonance (NMR) Spectroscopy
  • B. Chromatography
    • 1. High-Performance Liquid Chromatography (HPLC)
    • 2. Gas Chromatography (GC)
    • 3. Affinity Chromatography
  • C. Electrophoresis
    • 1. Polyacrylamide Gel Electrophoresis (PAGE)
    • 2. Agarose Gel Electrophoresis
    • 3. Capillary Electrophoresis
  • D. Centrifugation
    • 1. Differential Centrifugation
    • 2. Density Gradient Centrifugation
    • 3. Ultracentrifugation
  • E. Microscopy
    • 1. Light Microscopy
    • 2. Electron Microscopy
    • 3. Atomic Force Microscopy (AFM)
  • F. PCR and DNA Sequencing
    • 1. Polymerase Chain Reaction (PCR)
    • 2. DNA Sequencing
  • G. Protein Purification
    • 1. Cell Lysis and Extraction
    • 2. Column Chromatography
    • 3. Electrophoretic Techniques
    • 4. Ultrafiltration and Dialysis
  • H. Enzyme Assays
    • 1. Spectrophotometric Assays
    • 2. Fluorometric Assays
    • 3. Radiometric Assays
    • 4. Coupled Enzyme Assays
    • 5. Discontinuous Assays
  • I. Immunological Techniques
    • 1. Enzyme-Linked Immunosorbent Assay (ELISA)
    • 2. Western Blotting
    • 3. Immunoprecipitation (IP)
    • 4. Flow Cytometry
    • 5. Immunohistochemistry (IHC) and Immunofluorescence (IF)
  • Final words about Biochemical Techniques basics
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