Biochemistry Question Bank for Second B.Sc. Students

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This is academic year ending, examinations started. Students are preparing examinations. We are providing the questions lists, which is prepared from previous papers of the B.Sc. Second year. You can download this Biochemistry Question Bank in designed PDF format from below link.

Biochemistry Question Bank for Second B.Sc. Students

UNIT 1: Bioenergetics & Biological Oxidation

Essays: (15Marks)

1. What are High energy compounds? Write about few examples with suitable structure?
2. What is Free energy? Explain the free energy concept?
3. What is Electron Transport Chain? Explain the mechanism of ETC at mitochondrial Matrix?
4. What is Oxidative phosphorylation? Write about chemi-osmotic theory? Mention few examples of Inhibitors and uncouplers?
5. What is photophosphorylation? Explain cyclic and non cyclic photophosphorylation stages in light phage?

Short Questions: (4 Marks):

1. Cytochromes
2. Energy-rich compounds
3. Conformational coupling hypothesis
4. Inhibitors of ETC
5. Structure and Functions of Mitochondria
6. Flavoproteins (FAD and FMN)
7. Structure of Chloroplast
8. Chlorophyll-Structure and Function
9. Cyclic Photophosphorylation
10. Non-cyclicphotophosphorylation
11. Hill reaction
12. Hatch-Slack cycle
13. Photorespiration
14. Oxygenases
15. Redox reactions
16. Redox couplers

Very Short Questions: (2 Marks):

1. Structure of 2,4-DNP
2. Structure of NAD⁺
3. Free energy
4. Thermodynamic laws (First & Second Laws)
5. Mitochondria
6. Chloroplast
7. Cytochrome C
8. Terminal Oxidase
9. Cytochrome Oxidase

Reference Books:

1. Fundamentals of Biochemistry by J.L.Jain
2. Principles of Biochemistry by Lehninger, Nelson & Cox

Unit 2: Carbohydrate Metabolism & Lipid Metabolism

Essays: (15Marks):

1. Describe TCA cycle with its regulation?
2. Describe about HMP Shunt? Write about significance?
3. Describe the process of Glycogen Metabolism
4. Describe the Biosynthesis process of Fatty acids?
5. How the Fatty acids are oxidizes in the human body?
6. Write about β-oxidation of Fatty acids? Calculate the net ATP in this process by taking Palmitic acid is an example.
7. Write about the process of Biosynthesis of Cholesterol?
8. Write the steps involved in Glycolytic pathway (or) EMP pathway?

Short Questions: (4 Marks):

1. Triacylglycerol biosynthesis
2. Anaplerotic reactions (or) Anaplerosis
3. Gluconeogenesis
4. Glycogenesis
5. Glycogenolysis
6. Significance of HMP shunt
7. Unsaturated fatty acid oxidation
8. α-oxidation of Fatty acids
9. Ketone bodies (Ketogenesis & Ketogenolysis)
10. Net ATP calculation in β-oxidation of Fatty acids
11. Multienzyme complex & Multifunctional enzyme complex
12. Biosynthesis of USFA
13. Lipoproteins & types
14. Pyruvate dehydrogenase (PDH) complex & its mechanism
15. Lactate dehydrogenase (LDH) complex
16. Glycolysis
17. Substrate level phosphorylation.

 Very Short Questions: (2Marks)

1. Glycolysis
2. Anaplerosis
3. Structures: Glucose-6-P,Fruc-6-P, Fructose-1,3-bis P, Glyceraldehyde-3-P, 1,3-bisPG , 2,3-bisPG, DHAP, PEP, Pyruvate, OAA, , Acetyl~coA, Citric acid (or) Tricarboxylic acid, Isocitrate, Succinyl~coA, alpha-ketoglutarate, Malic acid, Fumarate (For this structures visit: Bio-Gallery)
4. Definitions: Metabolism, Anabolism, Catabolism, Anaplerosis, β-oxidation of Fatty acids, Cholesterol, bile acids, bile salts, (For this definitions Visit: Biochemistry Glossary)

Reference Books:

1. Fundamentals of Biochemistry by J.L.Jain
2. Principles of Biochemistry by Lehninger, Nelson & Cox
3. Fundamentals of Biochemistry by Voet & Voet

Unit 3: Nitrogen Compound Metabolism (Amino acids, Nitrogenous Compound Metabolism)

Essays: (15Marks)

1. Describe the denovo pathway of Purine Biosynthesis with its regulation?
2. Explain the biosynthesis process of Pyrimidine Biosynthesis with its regulation?
3. Describe the biosynthesis of Heme & its regulation?
4. Write the Biosynthesis of Tryptophan?
5. Write the Biosynthesis of Histidine?
6. Write the general reactions involved in Amino acid Metabolism

Short Questions: (4 Marks):

1. Purine catabolism
2. Pyrimidine catabolism
3. Branched chain amino acids catabolism.
4. Serine-Glycine interactions
5. Essential Amino acids & Non Essential Amino acids
6. Gout
7. Lesch- Nyhan syndrome
8. Orotic aciduria
9. Ribonucleotide reductase
10. Salvage Pathway
11. Transamination
12. Deamination
13. Decarboxylation
14. Thymidine Synthase

Very Short Questions: (2Marks)

1. Gout
2. Structure: XMP, IMP, UMP, CMP, ATP, cAMP, GMP, TMP, Homocystine, Homoserine, Chorismate, Shikimate, Phenylpyruvate, Tryptophan, Essential amino acids structures, Histidine, Bilirubin, Biliverdin, Heme
3. Essential amino acids
4. Non-Essential amino acids
5. Glycogenic and Ketogenic amino acids

Reference Books:

1. Fundamentals of Biochemistry by J.L.Jain
2. Principles of Biochemistry by Lehninger, Nelson & Cox
3. Fundamentals of Biochemistry by Voet & Voet

Unit 4: Biochemical Techniques

Essays: (15Marks)

1. Write the Principle, Procedure and Applications of Paper Chromatography
2. Describe the Principle, Procedure and Applications of SDS-PAGE?
3. Describe the Principle, Procedure and Applications of Gel-filtration?
4. Write about Ultra centrifugation (or) Analytical centrifugation and its Applications?
5. Explain Density gradient Centrifugation and its Applications?
6. Write the Application of RadioIsotopes in Medicine?
7. What is the Principle, Applications and Instrumentation of Spectrophotometry.

Short Questions: (4 Marks):

1. TLC
2. Ionic Exchangers (Catioinic & Anionic Exhangers)
3. Ion-Exchange Chromatography
4. Affinity Chromatography
5. Paper Electrophoresis
6. Differential Centrifugation
7. Colorimetry
8. RCF
9. Partition Coefficient (or) Distribution coefficient
10. Desktop & Analytical Centrifuges
11. Adsorption chromatography
12. Isoelectric focusing
13. Polyacrylamide gels
14. Homogenizer
15. Application of SDS-PAGE

Very Short Questions: (2Marks)

1. Isotope
2. Principles of Electrophoresis, Chromatography, Centrifugation and Spectroscopy (Any one from these)
3. Beer’s-Lambert’s Law
4. Β-mercaptoethanol & Urea
5. SDS
6. What is Half life
7. Optical Density (OD)
8. Radioactivity and its units
9. Electrolytes
10. Polyacrylamide gels
11. Agarose gels
12. Ionic Strength
13. Starch gels
14. Solubilizers

References of this Unit:

1. Biophysical Chemistry by Upadhyaya & Upadhyaya
2. Biotechniques Theory and Practical by SVS Rana
3. Principles and Practical of Biochemistry & Molecular Biology by Wilson & Walker

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